Calibration is independent of the speed.
It measures the size of a 5MB download via the graphing system, and via a HTTP request. The two should agree i.e. give 1.000.
If more than 2 then likely to be an AV issue, or multiple interfaces being monitored.
When you say speedtest which menu option exactly? The run thinkbroadband speedtest is not linked to the tool, but jumps off to a webpage and runs our usual web based speed test.
Try pressing CTRL SHIFT F2 when the main graph has the focus, this will run a test sequence.
shows the app when running with TCP/UDP disabled doing some lan file transfer tests